plant homogenizer
surface water chlorophyll A Tissue Grinding in accordance with the Ministry of Environmental Protection, the standard for chlorophyll a "Determination of Chlorophyll a in Water Quality Spectrophotometry" HJ 897-2017 has developed a new tissue grinder。
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surface water chlorophyll A Tissue Grinding in accordance with the Ministry of Environmental Protection, the standard for chlorophyll a "Determination of Chlorophyll a in Water Quality Spectrophotometry" HJ 897-2017 has developed a new tissue grinder。
Application of DHFSTPRP-Chlorophyll24 standard method for chlorophyll extraction:
1. Filtration: Add magnesium carbonate suspension before suction filtration to prevent pigment decomposition. The negative pressure cannot exceed 50kPa.
2. Chlorophyll extraction: place the filter on DHFSTPRP-Ch24In the extractor, add 10ml of 90% acetone solution and grind at 1500-2000rpm for 1min. Disrupt phytoplankton algal cells on the filter membrane. After grinding, soak in the dark at 4°C for extraction, and the soaking time is 15min to 24h. Final centrifugation for clarification: centrifuge at 1000 g for 20 min or filter and transfer the supernatant to a stopper glass vial.
3. Determination method: spectrophotometry, three-color equation to measure the absorbance at 664nm, 647nm, 630nm, 750nm to calculate the content of chlorophyll a, b, c chlorophyll . Monochromatic equations were used to determine the corrected content of pheophytin a.
DHFSTPRP-Ch24Rapid cell disruption, cell lysis, and tissue homogenization can simultaneously process 8×10mL centrifuge tubes, etc.
The three-dimensional integrated vibration mode makes the sample grinding more uniform , sufficient, good sample repeatability, no cross-contamination between samples, is a real high-throughput grinding and shaking system. Each sample only takes 1-2 minutes to complete.
Accessories are cryogenically ground and preserved.
DHFSTPRP-Ch24 Samples and applications:
1. PCR (polymerase chain reaction) rapid multi-sample grinding preparation
2. Rapid lysis of tissue homogenate , carry out nucleic acid extraction, RNA/DNA extraction, protein extraction
3. Lysis of yeast and bacterial cells
4. Pesticide/Insecticide Analysis (In LC/MS/MS testing food residues pesticides or toxic substances sample processing
5. QuEChERS method (pesticide, antibiotic, drug extraction)
6. APIs extraction of medicinal active ingredients
7. Food safety (contamination, adulteration, food traceability)
8. Biofuel grinding and homogenization
Surface Water Chlorophyll Tissue GrinderExperimental steps:
Surface Water Chlorophyll Tissue GrinderKey Features:
• A specially designed grinder is designed for the extraction of nucleic acid and protein analysis components in plant samples.
• High-throughput: can grind 8*10ml samples at the same time, and can be applied to various sizes of samples Grinding greatly improves work efficiency.
• Each sample only takes 1-2 minutes to complete.
• In order to ensure the reproducibility, validity and comparability of samples, the grinder can set the grinding time, grinding rate and number of cycles to ensure that each Consistency of the first grinding and the next grinding conditions, the maximum time is 99 minutes.
• Built-in two safety latches and a sample hatch interlock, extremely safe and reliable.
• Customized plan for normal and low temperature animal and plant grinding.
• Start and stop buttons allow the user to start or stop the grinding process at any time.
• Optional Ningbo lawson Cryogenic accessories perform cryogenic grinding and preservation of temperature-sensitive RNA and protein extraction samples.
Surface Water Chlorophyll Tissue Grinder Technical parameters:
◆ Application areas: tissue homogenization, grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, oscillation.
◆ 24 samples can be processed in 15 seconds, and 12-bit and 24-bit liquid nitrogen freezing adapters can be used.
◆ Compatible sample size: 24*(0.2-0.5ML) /24*2ML /8*(5-15)ML /4 *25ML /2*50ML, various specifications of grinding tubes can be customized arbitrarily.
◆ Touch screen display, easy and intuitive operation.
◆ Ten groups of experimental data can be stored, and according to different experimental samples, animal heart, spleen, lung, kidney, bone, skin, and hair patterns can be set.
◆ Mode cycle: According to the set experimental parameters, it can continuously cycle between several set parameters to further reduce the interference of human factors.
◆ Anti-vibration principle: DHFSTPRP-1 innovative anti-vibration principle, as well as a special three-dimensional vibration mode of up and down and left and right shaking, the sample is in the shape of 8 in space Three-dimensional movement, innovative grinding bead movement, ensure ideal sample processing and instant crushing effect.
◆ Feed size: no requirement, adjust according to the adapter
◆ Discharge particle size: ~5µm
◆ Number of grinding platforms (acceptable number of grinding jars) >2
◆ Fastening with automatic centering is
◆ Homogenization speed: 0—70 HZ/second, working time: 0 seconds-9999 minutes, user can set by themselves;
◆ Grinding ball diameter: 0.1-30mm
◆ Grinding ball material: alloy steel, chrome steel, zirconia, tungsten carbide, quartz sand;
◆ Speed up: reach a higher speed in 2 seconds
◆ Slow down: reach a lower speed in 2 seconds
◆ Noise level:less than 55db
◆ Grinding method: Wet grinding, dry grinding, and low temperature grinding are available
◆ Ability to upgrade to ultra-low temperature liquid nitrogen refrigeration or air cooler refrigeration
◆ Adapter material: PTFE or alloy steel
◆ Safety lock when the fastening device with automatic center positioning works, protects the whole process
◆ Grinding kit material is rigid, Teflon (Teflon) zirconia
◆ Dimensions: 630*300 *480mm
◆ Weight：60KG
Surface water chlorophyll tissue grinderExperiment results:
