Cryogenic Homogenizer

   DHFSTPRP-LN Rapid Cryogenic Freezing Homogenizer is specially used for grinding heat-sensitive substances, various animal and plant tissues, microorganisms, rubber, plastics, food, medicine, coal, oil shale, wax products, PE, PS, textiles, resins, etc. It is tough at room temperature and difficult to crush. substance.

    DHFSTPRP-LN Rapid Cryogenic Freezing Homogenizer the above substances are first frozen to below the embrittlement point, and then ground to the required fineness in a grinder, and the original ingredients will not be destroyed. In addition, flammable and explosive substances at room temperature can also be crushed at low temperature, and the effect is more significant.

    This instrument is also known as:tissue homogenizer,cooling homogenizer,cooling Tissuelyser,freezing homogenizer,freezing Tissuelyser.

Operation steps:

1. Manual operation

(1)   Manually immerse the adapter and grinding jar into the liquid nitrogen sample, and quickly cool down to -196 degrees. Prevent sample degradation or achieve changes in the physical properties of the sample.

(2)   Then put the adapter and grinding jar on the grinder. Cover the safety cover and lock the safety lock to operate.

2. automatic operation

(1)   Load the adapter and grinding jar onto the grinder. Cover the safety cover and lock the safety lock to operate.

(2)   Open the liquid nitrogen connection device, start the liquid nitrogen delivery/or open the air cooler (to achieve 0 degree refrigeration).

(3)   Turn on the liquid nitrogen control switch, and after the liquid nitrogen flows into the crushing tank, the physical cooling is carried out. In about a minute, it quickly cooled down to -192 degrees.

(4)   Turn on the grinding button to operate. In just 15 seconds, the object shatters instantly.

(5)   The flow of liquid nitrogen can be controlled throughout the process, and can be filled at any time during the operation.

Features :

A. High data accuracy and reproducibility

1. The sample is always at liquid nitrogen temperature during grinding.

2. The sample is cooled to -100°C within 1 minute, and can reach -196°C within 4 minutes and 30 seconds.

3. The sample bottle is in a fully closed state during the crushing process, and the sample is completely retained in the bottle to ensure the control of dangerous and important samples.

4. The sample bottles are sealed independently, and it is very convenient to clean after each break, avoiding cross-contamination between samples and external contamination.

B. Versatility, Humanized Design

1. The touch-screen display can set parameters such as grinding time, cycle period, and grinding frequency (optional).

2. Removable control panel (optional).

3. 10 programs can be stored (optional).

4. 1.5ML 2ML 5ML 10ML 25ML 50ML and other sample tubes can be installed for sample grinding.

C. Durability

1. There is only one moving part - the collider, which greatly reduces the mechanical stress and prolongs the service life of the equipment.

2. Strong technical support: Ningbo Luoshang Company has more than 500 users and specializes in the production of sample pretreatment instruments.

Grindable samples:

DHFSTPRP-LN Rapid Cryogenic Freezing Homogenizer a special vertical up-down integrated vibration mode is adopted, through the high-frequency reciprocating vibration, impact and shearing of grinding beads (zirconia, steel balls, glass beads, ceramic beads). achieve goals quickly. The ground samples are more complete and uniform, the sample repeatability is better, and there is no cross-contamination between samples.

1)   Have five systems, sample grinding system, liquid nitrogen storage system, liquid nitrogen control system, circuit control system, heat preservation and cold preservation system.

2)   Application fields: biology, food, agriculture, pharmaceuticals, chemical/synthetic materials, engineering/electronics, building materials, glass/ceramics.

3)   Automatic filling of liquid nitrogen: The flow of liquid nitrogen can be controlled throughout the process, and can be filled at any time during the operation.

4)   There are two operation modes of liquid nitrogen grinding: manual and automatic.

5)   Have a liquid nitrogen connection device, which is automatically transported from the storage system to the sample grinding system.

6)     Low temperature or liquid nitrogen grinding is possible: Yes, it can be connected to a liquid nitrogen tank (Type II) or an air cooler (Type I).

7)   The shells are all made of hard steel to avoid plastic covers, which will affect safety.

8)   Sample characteristics: hard, medium hard, soft,  brittle,  elastic, fibrous.

9)   Crushing principle: low temperature freezing force, mechanical impact force, physical friction force.

10)  Temperature range: -196℃-100℃

11)  Injection size: ≤ 8 mm

12)  Discharge particle size: 0~ 5 µm

13)  Standard machine processing sample quantity: 50ml*2 (can be customized)

14)  Grinding jar size: 1.5ml/2ml/5ml/10mL/25ml/35ml/50ml. Can be customized.

15)  When the grinding jar is placed, it can be done once without the need for repeated placement, which prolongs the working time and affects the temperature of the sample.

16)  Grinding speed: 1-70HZ adjustable (30-2100 rpm/100-7000RPM).

17)  Anti-vibration principle: DHFSTPRP-1 innovative anti-vibration principle, as well as a special three-dimensional vibration mode of up and down and left and right shaking, the sample moves in a three-dimensional figure-8 shape in space, which can be suitable for cracking various animal and plant tissues and microorganisms, which is convenient and fast The extraction of DNA, RNA and protein.

18)  Typical liquid nitrogen grinder: 15 seconds

19)  Grinding jar material: stainless steel, PTFE (Teflon)

20)  Grinding ball material: alloy steel, chrome steel, zirconia, tungsten carbide, quartz sand

21)  Acceleration: Reach maximum speed in 2 seconds

22)  Slow down: Reach lower speed in 2 seconds

*  The matching LAWSON kit can be used to quickly, efficiently, stably and batch extract DNA/RNA proteins from samples, etc.

*  1HZ=30 RPM  1HZ=100RPM

*  Conventional adapters can be placed in the refrigerator to give samples a cold environment while working. It can be equipped with an adapter with refrigeration, which can be soaked in liquid nitrogen for more than 30 minutes.

*  The noise emission value for the working environment depends on the type of sample and the settings of the grinder. The parameters in the table are in the no-load state

*  Small samples typically used for DNA and RNA isolation can be prepared in single-use centrifuge tubes. Adapters made of teflon material can be used to hold 5 or 10 single-use sample tubes for cell disruption. Cells and tissues in the sample tissue grinder are rapidly and efficiently disrupted in a very short period of time, thus eliminating the need for additional cryogenic refrigeration.

Rapid Cryogenic Freezing Homogenizer DHFSTPRP-LN is specially used for grinding heat-sensitive substances, various animal and plant tissues, microorganisms, rubber, plastics, food, medicine, coal, oil shale, wax products, PE, PS, textiles, resins, etc. It is tough at room temperature and difficult to crush. substance.

Rapid Cryogenic Freezing Homogenizer the above-mentioned substances are first frozen to below the embrittlement point, and then ground to the required fineness in a grinder, and the original ingredients will not be destroyed. In addition, flammable and explosive substances at room temperature can also be crushed at low temperature, and the effect is more significant.