Non-contact ultra processor

LAWSON98-III non-contact ultrasonic crusher, also called cup crusher, is used for aseptic crushing. It can break chromosomes and break cells through the centrifuge tube. Suitable for ChP, ChP-seq, RNA-seq, and chromatin shearing and DNA shearing (for next-generation sequencing). It is specially tailored for the pretreatment of next-generation sequencing DNA samples and chromatin immunoprecipitation experiments. Compared with the traditional probe-contact ultrasonic cell crusher, non-contact samples can be crushed in a sealed container without causing infectivity. Flying fog, the ultrasonic probe does not contact the sample to avoid cross-contamination. Non-contact ultrasonic pulverizers can achieve unparalleled quality, efficiency and safety of traditional ultrasonic methods.

Multiple samples can be detected at one time, with high experimental efficiency; no wear and slag removal, each sample is in a separate fully enclosed test tube to avoid cross-contamination; optional cooling water circulation system, which is convenient for samples to be ultrasonicated in a 4 ℃ water bath, energy The distribution is uniform and the ultrasonic effect is complete; the ultrasonic parameter setting is flexible, the experimental steps are standardized, the experiment repeatability is good, and the results are highly reliable.

It has gradually become an indispensable standardized tool for ChIP (chromatin immunoprecipitation) and DNA shearing research platforms. It has high experimental efficiency, reliable results, and good repeatability. It can process a minimum of 5ul samples and is suitable for precious samples.

The working principle of the non-contact ultrasonic cell crusher:

    The non-contact ultrasonic cell pulverizer (ultrasonic cell pulverizer) adopts the design of installing an ultrasonic generating device at the bottom of the water tank. In the traditional probe ultrasonic pulverizer, the micro-flow phenomenon caused by ultrasonic waves can only be generated near the probe. The non-contact ultrasonic cell pulverizer is equipped with an ultrasonic generator at the bottom of the water tank, so that the water tank is completely within the action range of ultrasonic waves, and the ultrasonic action is widely distributed and balanced, reducing the formation of foam. During the experiment, the non-contact ultrasonic cell pulverizer automatically and continuously rotates the centrifuge tube to make the ultrasonic power distribution more uniform. The non-contact ultrasonic cell pulverizer is in the middle of the experimental process. The samples are placed in independent fully sealed centrifuge tubes, and there is no cross-contamination between samples, which avoids the spread of aerosols.

Advantages of non-contact ultrasonic cell crusher:

    In the traditional ultrasonic cell pulverizer, the probe is in direct contact with the sample, and there is metal ion contamination. Only one sample can be processed at a time, and the experimental period is long; for multiple samples, the same probe needs to be reused, which is easy to cause cross-contamination of samples. Since the depth of the probe inserted into the sample is different each time, the energy distribution of each ultrasonic wave is also different, which affects the repeatability and accuracy of the experimental results. In addition, since a closed system cannot be used, the aerosol or foam generated during the ultrasound process can diffuse into the environment, creating a potential biohazard. The non-contact ultrasonic cell crusher can detect 4-32 samples at the same time, with high experimental efficiency; no need to operate the probe frequently, each sample is in a separate fully enclosed test tube to avoid cross-contamination; 4-degree water bath is used, ultrasonic energy distribution Uniform and complete ultrasonic effect; flexible ultrasonic parameter settings, standardized experimental steps, good experimental repeatability, and high reliability of results.

⒈ No aerosol aerosol production - enhanced biological safety (such as mycobacteria, viruses, etc.)

⒉ Eliminates the risk of cross-contamination of samples

⒊ Eliminate the phenomenon of traditional probe wear and slag

⒋Can handle a variety of samples, a wide range of sample processing

⒌Applicable to various standard containers

⒍Can be used to process micro samples, the minimum is 5ul

⒎The automatic continuous rotation of the centrifuge tube makes the energy distribution of the ultrasonic wave more uniform

⒏ Optional high and low temperature constant temperature water bath, customized Eppendorf tube crushing rotating base of various diameters according to customer requirements

Application range:

⒈ DNA fragmentation

⒉ RNA fragmentation

⒊Bacteria and cell disruption

⒋ChIp assay (chromatin immunoprecipitation)

⒌Sample pretreatment for high-throughput sequencer

⒍ Take membrane protein

⒎ Homogenization, emulsification reaction

⒏ Ultrasonic treatment of precious reagents

2018 new non-contact ultrasonic crusher LAWSON98-III product advantages:

In closed systems, the aerosol or foam generated during sonication can diffuse into the environment, creating a potential biohazard. The non-contact automatic ultrasonic crusher can detect 4-32 samples at a time, with high experimental efficiency; no need to operate the probe frequently, each sample is in a separate fully enclosed test tube to avoid cross-contamination; ultrasonic energy distribution is uniform, and ultrasonic effect Complete; flexible ultrasonic parameter settings, standardized experimental steps, good experimental repeatability, and high reliability of results.

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PDF:Cell Disruption

Methods of cell disruption:

1. Mechanical crushing method: refers to the use of a masher, a grinder or a homogenizer to break the cells apart.

1. High-speed tissue mashing: make the material into a thin paste liquid, place it in about 1/3 of the volume of the cylinder, close the cylinder lid tightly, turn the governor to the slowest position first, turn on the switch, and gradually accelerate to the desired position. speed required. This method is suitable for animal visceral tissue, plant succulent seeds, etc.

2. Homogenization by glass homogenizer: first place the chopped tissue in a tube, then insert the grinding rod into the tube to grind it back and forth, and move it up and down to grind the cells. High, suitable for small amount and animal organ tissue.

2. Physical fragmentation method: refers to the use of temperature difference, pressure difference or ultrasonic wave to break cells apart.

1: Treat the cell suspension with a certain power of ultrasonic wave, so that the cells are violently shaken and broken (the cell wall and organelles are broken by the vibration force of the ultrasonic wave).

Mechanism: It may be related to the cavitation phenomenon of bubble generation, growth and fragmentation when strong sonic waves act on the solution, and the shock wave and scissor force caused by the cavitation phenomenon cause cell lysis.

The efficiency of sonication depends on the sound frequency, sound energy, treatment time, cell concentration and cell type, etc. (Pay attention to cooling down when using to prevent overheating).

2. High-pressure fragmentation: The cell suspension is ejected from the annular gap of the high-pressure chamber onto the stationary impact ring, and is forced to change direction and flow out through the outlet tube. During this process, the cells undergo high-speed shear collisions and changes from high pressure to atmospheric pressure, thereby breaking up and releasing the contents.

This is an ideal method for gentle, thorough cell disruption.

3. Repeated freeze-thaw method: freeze the cells below -20 degrees, thaw at room temperature, and repeat several times. Due to the formation of ice particles in the cells and the increase of the salt concentration of the remaining cell fluid, the swelling will be caused, and the cell structure will be broken.

3. Chemical fragmentation method: refers to the use of formaldehyde, acetone and other organic solvents or surfactants to act on the cell membrane, so that the structure of the cell membrane is damaged or the permeability is changed.

Some animal cells, such as tumor cells, can be destroyed by the use of sodium dodecyl sulfonate (SDS), sodium deoxycholate and other cell membranes. The concentration is generally 1 mg/ml.

4. Enzymatic fragmentation method: Use appropriate enzymes to destroy the cell wall, and then fragment the protoplasts in a hypotonic solution.

Bacterial cell walls are thicker and can be treated with lysozyme for better results.

Standard formulation of lysate: : 50mM Tris-HCl (pH8.5~9.0), 2mM EDTA, 100mM NaCl, 0.5% Triton X-100, 1mg/ml lysozyme. (Lysozyme works better in this pH range).

Comprehensive description: No matter which method is used to disrupt tissue cells, intracellular proteins or nucleic acid hydrolase will be released into the solution, which will biodegrade macromolecules, resulting in a reduction in the amount of natural substances. Adding diisopropyl fluorophosphoric acid (DFP) Can inhibit or slow down autolysis; the addition of iodoacetic acid can inhibit the activity of those proteolytic enzymes that require a sulfhydryl group in the active center, and the addition of phenylmethanesulfonyl fluoride (PMSF) can also eliminate the activity of proteolytic enzymes, but not all, And it should be added several times while crushing; in addition, pH, temperature or ionic strength can also be selected to make these conditions suitable for the extraction of the target substance.

Precautions before use:

1. Select an appropriate probe according to the volume of the sample to be processed. Before and after use, the probe must be wiped with an alcohol cotton ball, and the probe must be replaced with a special wrench;

2. When the AMPLITUDE knob is turned on, the probe must not be exposed to the air, and in special cases (test probe) should not exceed 10 seconds;

3. Prepare an ice box before use, and the sample must be placed in an ice bath during processing, and the sample concentration should not be too large.

Non-Contact Ultrasonic Crusher Lawson98-III is used for aseptic breakage, which can break chromosomes and break cells through the centrifuge tube. With silencing and pressing device, DL-1510 type low temperature coolant circulation machine is optional.

Non-Contact Ultrasonic Crusher is often used for aseptic breakage. It can break chromosomes and break cells through the centrifuge tube. The compacting device with silencer is especially used for the release of adenovirus particles. In addition to being suitable for the preparation of high-titer recombinant adenovirus, it can also prepare viral DNA and DNA terminal protein compounds, and is an ideal instrument for soil sample preparation. It has become an indispensable standardization tool for CHIP (chromatin immunoprecipitation) research platforms. Non-contact ultrasonic cell pulverizer, also known as ultrasonic cell pulverizer, is suitable for reaction systems with special needs. Compared with traditional contact ultrasonic methods, it has outstanding performance in terms of safety, precision, reaction efficiency, reaction boundary conditions and reaction uniformity. The advantages. Non-contact ultrasonic cell pulverizers are widely used, mainly for cell disruption, lysis and release of cell content particles.