WELCOME TO LAWSON!
LAWSON

Keywords:ultrasonic processor,homogenizer,Vortex mixer

LAWSON

News

News
Position: Home > News > Technical

Technical

LAWSON08-III Process and reagent instrument for breaking and shearing DNA genome

Article source:LAWSON Author:Lawson Popularity: 1656 Issuing Time:2018/6/8 9:14:12

Ultrasound disrupts genomic DNA

(1) Quantification of genomic DNA: 1 μg of genomic DNA (37.5yl system)

(2) Ultrasound interrupts genomic DNA: 37.5pul genomic DNA is interrupted to 200bp by ultrasound

left and right fragments

1. Ultrasonic working conditions: Ultrasonic 30s interval 30s, take out samples every 10 cycles,

Vortex and mix, put back and continue to sonicate, three times in total.

2. Detection of ultrasonic interruption by agarose gel electrophoresis

 

List of reagents and instruments required for genomic DNA fragmentation

1. Water for construction of the reservoir

2. Non-contact ultrasonic breaker (LAWSON ultrasonic breaker) model: LAWSON08-III

3. Vortex mixer VORTEX model: DH-901

4. Handheld centrifuge model: LX-100

5. Pipette brand eppendorf  (10p 1 20u l 100p l)




    Copyright © 2003~2023 Ningbo Lawson Smarttech Co.,Ltd | Lawson·China | 浙ICP备15028117号-8