Technical

Ultrasound disrupts genomic DNA

(1) Quantification of genomic DNA: 1 μg of genomic DNA (37.5yl system)

(2) Ultrasound interrupts genomic DNA: 37.5pul genomic DNA is interrupted to 200bp by ultrasound

left and right fragments

1. Ultrasonic working conditions: Ultrasonic 30s interval 30s, take out samples every 10 cycles,

Vortex and mix, put back and continue to sonicate, three times in total.

2. Detection of ultrasonic interruption by agarose gel electrophoresis

List of reagents and instruments required for genomic DNA fragmentation

1. Water for construction of the reservoir

2. Non-contact ultrasonic breaker (LAWSON ultrasonic breaker) model: LAWSON08-III

3. Vortex mixer VORTEX model: DH-901

4. Handheld centrifuge model: LX-100

5. Pipette brand eppendorf  (10p 1 20u l 100p l)