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Advantages and disadvantages of ultrasonic cell crusher

Article source:LAWSON Author:LAWSON Popularity: 934 Issuing Time:2018/2/28 13:29:15

During the development process of the ultrasonic cell crusher, it has the characteristics of high cell activity crushing, which improves the sample processing efficiency and the bacterial cell crushing rate. The crushing rate of the bacterial cells can reach more than 99% after one sample treatment. And the physiological activity of the target protein is protected to the greatest extent, and some preliminary results have been achieved.

Ultrasonic cell crusher uses ultra-high pressure energy to crush cells. Compared with ultrasonic waves, the advantages of ultrasonic cell disintegrators are: maintaining cell viability, saving time, less sample loss, and can process a large number of samples. Therefore, the continuous development and improvement of ultrasonic cell pulverizers have made a considerable contribution to large-scale production.

In the biotechnology industry, the cost of separation and purification often accounts for 60% to 90% of the cost of the entire process. my country's biotechnology industry is dominated by the traditional fermentation industry. Product separation and purification is the most important stage of the fermentation industry, and it is the downstream of the entire fermentation production line, which not only determines the yield, but also determines the quality and safety of the product. This part includes many operations: separation of cells and fermentation broth, breaking cells to release intracellular products, membrane separation to remove cell debris, etc.

Ultrasonic crushing by ultrasonic cell crusher is the most commonly used crushing method in biological production. Fermentation broth of different strains. The effect of ultrasonic fragmentation varies greatly. In general, bacilli are more fragile than cocci, gram-negative bacterial cells are more fragile than gram-positive bacterial cells, and less effective against yeast. Advantages: Easy to operate, less fluid loss, suitable for laboratory scale. Disadvantages: high cost; easy to cause a sharp rise in temperature; in large-scale operations, sound energy transfer and heat dissipation are difficult, and the generated chemical radicals are easy to deactivate the product.

There are many manufacturers producing ultrasonic cell crushers, and the technological content of each other's products is different, which directly leads to the difference in the service life of ultrasonic cell crushers. How long can the ultrasonic cell crusher be used? Let's follow Shanghai together. Let's take a look at the question of how long the ultrasonic cell disruptor can last.

How long can the ultrasonic cell crusher be used? This is actually a difficult question. How long the ultrasonic cell crusher can be used is related to many factors. It is not only related to the quality of the ultrasonic cell crusher, but also to the operation of the ultrasonic cell crusher. Relationship. Generally speaking, the better the quality of the ultrasonic cell crusher, the longer its corresponding use time, and the opposite is shorter; (for example, the general service life of the ultrasonic cell crusher produced by our Dihao-DIHOO is 3 to 5 years. look).

The daily use of the ultrasonic cell crusher will also affect its lifespan. Generally, those that are properly maintained and operated will generally be used for a longer time. On the contrary, the service life of the ultrasonic cell crusher will be reduced, so we are using ultrasonic cells. During the process of the crusher, you must read its instructions first, and then use it, so that the ultrasonic cell crusher we bought can be used for a longer time.

If you still don't know something about the ultrasonic cell crusher, you can conduct online consultation and one-to-one communication with our technology (www.csb17.cn).


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